Method Profile
Follow tumor-cell phagocytosis, tissue infiltration and tumor depletion by microglia obtained from human cortical organoids.
Preclinical functional evaluation of an EGFRvIII-directed myeloid-cell therapeutic approach.
Human cortical organoid-derived microglia, glioblastoma cells and combined glioblastoma–cortical organoid models.
Two-dimensional phagocytosis assays are paired with longer observation in three-dimensional tumor–brain models.
Effector cells
Cortical organoid-derived microglia expressing an EGFRvIII-directed CAR.
Tumor models
Two-dimensional glioblastoma cocultures and three-dimensional glioblastoma–cortical organoid models.
Molecular analysis
Single-cell RNA sequencing of phagocytic pathways and internalized tumor transcripts.
Evaluate the 2D and 3D functional observations separately, then connect them with the single-cell measurements.
Two-dimensional coculture followed by three-dimensional tumor–brain organoid evaluation.
Human cortical organoid-derived microglia and glioblastoma model systems.
Two-dimensional and three-dimensional experimental contexts.
CAR microglia selectively phagocytosed tumor cells in 2D and infiltrated tumor regions with tumor depletion in 3D.
Microglia obtained from human cortical organoids were directed against EGFRvIII. Two-dimensional and three-dimensional experiments followed phagocytosis, tumor-site infiltration and tumor-cell depletion.
