Method Profile
Tissue size, ATP, proliferation, apoptotic staining and contraction are followed after 7- or 14-day preservation and defined recovery periods.
Characterization of model handling and tissue-quality recovery before subsequent cardiac research.
Cardiac organoids from one hiPSC line, selected after initial comparison with immortalized-cell cardiac spheroids.
Short-term room-temperature transport-medium storage versus slow-freezing, followed by standard culture and serial recovery measurements.
Human tissue
Single-line hiPSC-derived cardiac organoids with unpreserved controls.
Preservation conditions
CellShip room-temperature medium and CryoStor CS10 slow-freezing, each tested over 7 or 14 days.
Measurements
ATP, size, Ki67/cleaved-caspase-3 and contraction timing, including beat-rate-normalized analysis.
Evaluate each endpoint over recovery time. Raw and frequency-normalized contraction parameters need separate interpretation.
Compare matched short preservation durations and several recovery intervals with unpreserved tissue.
One hiPSC background; figures report two to three biological replicates, with organoid-level sampling inside each experiment.
Unpreserved cultures sampled at the stated recovery-associated time points, and the alternative preservation condition.
Seven-day ambient storage supported earlier beating recovery and retained ATP; longer storage delayed recovery. Some morphology and frequency-normalized timing changes persisted or appeared later.
An academic preservation study compared short-term ambient storage with slow-freezing in human cardiac organoids. Room-temperature cultures retained ATP-associated viability, but recovery time and storage duration affected their contraction and injury measurements.
