Method Profile
Donor background and fatty-acid stress are compared in multicellular liver organoids before and after acetaminophen exposure.
Investigation of disease-background susceptibility and connected redox, mitochondrial and cell-injury responses.
iPSC-derived liver organoids containing hepatocyte-like, biliary, stellate, endothelial and Kupffer-like populations; two control and two MASH donor lines.
Air–liquid-interface tissue generation; oleic-acid challenge and acetaminophen response assessed with imaging, expression and functional assays.
Tissue and exposures
Control/MASH donor-derived organoids; oleic acid at 100 μM and acetaminophen dose comparisons at 5, 25 and 50 mmol/L.
Measurements
Lipid imaging/lipidomics, redox probes, mitochondrial potential, ATP and apoptosis-associated staining.
Keep donor-background, fatty-acid and drug comparisons explicit. Lipidomics uses one line per group across three differentiations and is distinct from the broader model-characterization panel.
Compare control and MASH cultures with and without fatty-acid pretreatment and subsequent acetaminophen challenge.
Four donor-derived cell lines for generation; lipidomics uses one control and one MASH line in three independent differentiations.
Matched untreated/vehicle and fatty-acid/drug conditions; published biopsy lipid data provide an external contextual comparison.
MASH-background and fatty-acid-pretreated cultures showed stronger selected oxidative, antioxidant and injury changes after acetaminophen.
Multicellular liver organoids derived from control and MASH donors differed in lipid accumulation and responses to acetaminophen. The study combines donor background, fatty-acid challenge and tissue-level injury measurements.
