Method Profile
Computational oxygen estimates and proximal-tubule measurements connect device permeability with injury and CDDO concentration responses.
Assessment of culture-environment control and platform-specific pharmacology in renal ischemia–reperfusion modeling.
Human RPTEC/TERT1 proximal-tubule cells in matched PET/PDMS double-layer chips and conventional inserts.
Glucose-free static low-oxygen interval followed by reoxygenation and restored chip flow; CDDO comparison at the 1% nominal oxygen setting.
Culture systems
PET/PDMS chips with matched geometry, microporous membranes and insert reference cultures.
Exposure characterization
Finite-element oxygen-transport/consumption simulation with morphology and viability observations.
Drug measurements
CDDO concentration response, CCK-8 viability and selected insert-derived injury/hypoxia gene markers.
Keep simulated oxygen separate from direct measurements and identify the actual platform used for each biological endpoint.
Compare injury across materials and CDDO responses between inserts and PET chips under the same nominal 1% oxygen condition.
One immortalized human proximal-tubule line; at least three independent injury/drug experiments.
In-air controls, PET/PDMS materials, static inserts and injured cultures without CDDO.
PDMS cultures showed little corresponding injury while PET chips developed marked viability loss. CDDO significantly improved insert viability at 100 nM; PET showed an improving trend up to 1 μM, with higher-concentration toxicity in both systems.
Matched-geometry chips made from PET or PDMS produced different injury patterns under the same nominal oxygen conditions. Simulations and human proximal-tubule experiments linked platform oxygen transport with the observed drug response.
