Method Profile
Compare culture feasibility and tumor-marker preservation in patient-derived renal cancer organoids connected with clinical registry data.
Develop and characterize a clinically linked preclinical tumor-model resource.
Twelve established renal tumor cultures from an 18-patient sampling study; five cultures received histological characterization.
Seven culture conditions compared through persistence, passage history, microscopy and matched tissue analysis.
Source tissue
Fresh or cryopreserved nephrectomy material with confirmed clear cell carcinoma for subsequent analyses.
Culture formats
Basement membrane matrices and a collagen-based air–liquid interface comparison.
Characterization
Matched histology and CA-IX/CK7 staining, linked with UroCCR clinical records.
Report enrollment, established cultures and histologically characterized cultures as separate stages; compare each culture with its matched source tumor.
Feasibility and matched histological characterization.
18 enrolled patients, 12 established cultures and 5 histologically characterized cultures.
Seven culture conditions and corresponding primary tissue.
Median culture duration was 52 days; marker expression varied, including CK7 differences from primary tissue.
A Caen feasibility study compared seven culture conditions for clear cell renal cell carcinoma. The model collection was connected with a clinical registry, while tissue characterization revealed variable marker preservation.
